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Sense Strand
Antisense Strand
Concentration (nM)
Target Gene
EGFP
ApoB
SSB
Transfection Method
Interfer (Polypus Transfection)
Lipofectamine 2000
Lipofectamine RNAiMAX
Cell Line
HeLa
HEK 293
Hepa 1-6
Measurement Method
Luciferase Reporter Assay
Dual Luciferase Assay
Real-time PCR
Predict Efficiency
Top-5 Candidates
Rank
Modification
Efficiency
Top-1
3rd pos: Methoxy, 5th pos: Methoxy, 4th (antisense): Fluoro
90%
Top-2
...
88%